Extended Data Fig. 1: In vivo RNAi screen to identify CSPs required for synaptic partner matching.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
a–c, Confocal images of adult antennal lobes showing neuropil staining by N-cadherin antibody (blue) and VA1d-ORN axons (green). The VA1v glomerulus is outlined based on N-cadherin staining. Control VA1d-ORN axons only innervate the VA1d glomerulus dorsal to the VA1v glomerulus (a). Some VA1d-ORN axons mistarget to the VA1v glomerulus in Ptp10D RNAi expressed from all ORNs (b, maximum projection of 3 sections with 1-µm interval), or Toll2 RNAi expressed in all PNs (c). d–h, Confocal images of adult antennal lobes showing neuropil staining by N-cadherin antibody (blue) and VA1v-ORN axons (green). The VA1d glomerulus is outlined based on N-cadherin staining. Control VA1v-ORN axons only innervate the VA1v glomerulus ventral to the VA1d glomerulus (d). Some VA1v-ORN axons mistarget to the VA1d glomerulus in kek1 RNAi expressed in all ORNs (e), kirre RNAi expressed in all ORNs (f), hbs RNAi expressed in all neurons (g), or sns RNAi expressed in all neurons (h). i, Penetrance of the mistargeting phenotypes in a–h. n refers to total antennal lobes examined. Scale bars = 10 µm.
