Extended Data Fig. 4: Characterization of new genetic drivers.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
a, Schematic for VA1d-ORN sparse drivers using a previously described method67. The transcription activation domain (AD) is controlled by the enhancer GMR78H05 and gated by FRT100-STOP-FRT100. FRT100 sites are ∼1% efficient as of wild-type FRT sites. STOP represent a transcription termination sequence. Heat-shock-induced FLP expression often enables split GAL4 expression in a single VA1d-ORN. b–d, VA1d-ORN sparse driver enable single VA1d-ORN labeling (green, labeled by a membrane-targeted GFP) as shown in the maximum projection (b), single contralateral section (c), or single ipsilateral section (d). The VA1d glomerulus location is verified by neuropil staining of N-cadherin (NCad) antibody (blue). e, Schematic of VA1v-ORN axons and VA1v-PN dendrites matching in the VA1v glomerulus (grey) in adult Drosophila antennal lobe (black solid line). Green, VA1v-ORNs; Magenta, VA1v-PNs. f, g, Single-cell RNA sequencing data showing the expression level of CG11317 in VA1v-ORNs (f) and Dop1R2 in VA1v-PNs (g) are consistently high across developmental stages. Heat map units: log2(CPM + 1). CPM: counts per million reads. Data adapted from previous published studies3,4. h, i, Gene-based genetic drivers73 express in VA1v-ORNs (green, labeled by a membrane-targeted GFP) (h) or VA1v-PNs (magenta, labeled by a membrane-targeted GFP) (i) at 30 h APF (left), 48 h APF (middle) and in adults (right). The VA1v glomerulus location is verified by neuropil staining of N-cadherin (NCad) antibody (blue). Both drivers enable expression of the transgenes in VA1v-ORNs or VA1v-PNs across developmental stages. Although they also express in ORNs or PNs targeting several other glomeruli, the expression is not detectable in glomeruli adjacent to VA1v (especially VA1d). *, PN cell bodies. Scale bars = 10 µm.
