Extended Data Fig. 9: Impaired depth perception and decreased PNN density in adult CCN1 cKO mice.
From: Astrocyte CCN1 stabilizes neural circuits in the adult brain
a. Exploration index in 1 min bins. Two-way ANOVA with post-hoc Tukey tests. b. Total distance traveled by mice during full 5 min of trial. Unpaired T-test. Symbols, mice. a,b. WT, n = 8. CCN1 cKO, n = 13. c. Representative single channel z-projections of WFA and PV staining. Same images as in Fig. 3r. Scale bar, 10 µm. d. WFA integrated density quantified per WFA+ cell. WT no MD n = 493 cells. WT MD n = 506 cells. cKO no MD n = 474 cells. cKO MD n = 446 cells. 5 mice/group. e. Representative z-projection of IBA1, WFA, and CD68 staining in adult VC. Scale bar, 10 µm. f. WFA colocalized to CD68+ puncta within IBA1 volumes. g. IBA1 volume within each field of view (FOV). h. Representative z-projection of IBA1and CD68 staining in adult VC. Scale bar, 10 µm. i. Volume of CD68+ puncta within IBA1 volume. N = 39-40 microglia/group from 4 mice/group. d-i. Kruskal Wallis tests with post-hoc Dunn’s tests on cells. j. Representative z-projection of IBA1 staining from adult VC, top. Representative cell body and process tracing (skeleton), bottom. Scale bar, 10 µm. WT no MD, n = 883 microglia, 4 mice. WT MD, n = 612 microglia, 4 mice. cKO no MD, n = 667 microglia, 4 mice. cKO MD, n = 543 microglia, 3 mice. k. Proportion of microglial morphotypes. Black symbols, mouse averages. Box plots, median with upper/lower quartiles. Whiskers, full range of data. Two-way ANOVA with post-hoc Tukey’s tests on mouse averages. l. Form factor. m. Total skeleton length. n. Mean branch length. o. Soma eccentricity. l-o. Kruskal Wallis test on cells with post-hoc Dunn’s tests. Bar graphs, mean ± SEM. Violin plots, median, upper and lower quartiles. For all dot plots, dots represent individual cells. Lines and bars, median ± 95% CI.
