Extended Data Fig. 1: Bulk ribo-Seq of visual cortex astrocytes.
From: Astrocyte CCN1 stabilizes neural circuits in the adult brain
a. Upset plot showing up- and down-regulated genes across the different comparisons (MD con vs ipsi, DR vs NR, CP vs Adult). MD, monocular deprivation. Con, contralateral eye to deprivation. Ipsi, ipsilateral eye to deprivation. CP, critical period. Adult, P120. DR, dark rearing. NR, normal rearing. 2 mice pooled per biological replicate. P28, n = 5. P120, n = 6. MD con/ ipsi, n = 3. P45 DR, n = 4. P45 NR, n = 3. Statistics done with DESeq2 using HOMER on biological replicates, Benjamini-Hochberg’s corrections for multiple comparisons. p-adjusted <0.05. b. Gene-set enrichment analysis (GSEA) results using the Reactome Database. NES is normalized enrichment score. Cutoff of adjusted p < 0.05 with a Benjamini-Hochberg correction. Pathways were significant in at least two comparisons. c, d. Overrepresentation analysis (ORA) to determine Gene Ontology (GO) terms enriched in DEGs across the comparisons. BP: Biological Process, CC: Cellular Component, MF: Molecular Function. Cutoff of adjusted p < 0.05 with a Benjamini-Hochberg correction.
