Extended Data Fig. 2: Identifying CCN1 as a pro-stability astrocyte factor.
From: Astrocyte CCN1 stabilizes neural circuits in the adult brain
a. Heatmaps showing FPKM values and Log2FC of astrocyte-expressed synaptogenic, synapse elimination, and extracellular matrix genes. Stars, significance. P-adjusted <0.05. b. Predicted functional protein network of CCN1 using STRING database. c. Tiled sagittal brain section of an adult mouse smFISH for Ccn1. Scale bar, 1 mm. d. Representative z-projection of P120 smFISH in a tile image of the visual cortex (VC). smFISH against Slc1a3, Tubb3, and Ccn1. Same image from Fig. 1g. e. Ccn1 relative area per astrocyte after dark-rearing to P45 (DR) or normal rearing to P45 (NR) in the VC. Two-way ANOVA with post-hoc Tukey tests on mouse averages. Small dots, individual astrocytes. Large circles and bars, mouse averages ± SEM. N = 3 mice/ group.
