Extended Data Fig. 9: Single-cell RNA-seq analysis of Uba1M41T/y HSPCs.
From: Independent mechanisms of inflammation and myeloid bias in VEXAS syndrome
a, Reduced dimensionality projection and joint clustering of HSPCs from all genotypes and timepoints. Expression of representative genes from each major annotated cell type is presented. b, Annotation of cells by M41T status, as determined, where possible, directly from sequencing reads mapping to the 5’ end of Uba1. c, Gene set module scores, stratified by genotype and timepoint post electroporation. d, Percent M41T editing in ex vivo HSPC cultures quantified directly from scRNA-seq reads. e, Reduced dimensionality projection and joint clustering of HSPCs separated and coloured by indicated genotypes and timepoints. Statistical test: two-sided Mann Whitney Test. **P < 0.01, ***P < 2.2e-16. Boxplots: centre line, median; box limits, first and third quartiles; whiskers, 1.5× interquartile range. In panel d, dots represent mean values and error bars represent SEM. 3 biological replicates per genotype were pooled and then analysed for each timepoint.
