Fig. 1: Effects of FDX2 and FXN concentration on the kinetic rate of [2Fe–2S] cluster synthesis.
From: Cross-regulation of [2Fe–2S] cluster synthesis by ferredoxin-2 and frataxin
a, Kinetic rate of [2Fe–2S] synthesis monitored by UV-visible spectroscopy for the recombinant human ISC machinery across a titration of FXN (from 0 to 50 eq.) at discrete levels of FDX2 as indicated, measured in absorbance units (AU) (at 456 nm) per min. b, Re-ordered data from a, showing rates of [2Fe–2S] synthesis measured through a titration of FDX2 at discrete levels of FXN. c, Magnification of the rate of [2Fe–2S] synthesis across a titration of FDX2 in the absence of FXN (0 eq.) from b. The maximum rate of [2Fe–2S] synthesis per discrete level of FDX2 (a) or FXN (b,c) is highlighted as a green bar. Data in a–c are mean ± s.d., n = 3 independent experiments.
