Extended Data Fig. 6: Quantification of Fdx1 and Fdx2 transcripts and survival of frataxin-deficient flies with or without RNAi-induced Fdx1 knockdown.
From: Cross-regulation of [2Fe–2S] cluster synthesis by ferredoxin-2 and frataxin
a,b, Quantification of (a) Fdx1 and (b) Fdx2 transcript levels following RNAi-induced Fdx1 knockdown with the constitutive daGAL4 driver. Relative quantifications were performed by RT–qPCR on whole-body extracts of third instar larvae. c, Mean lifespan of fh-GAAs male flies. RU treatments did not affect the fly survival. d,e, Survival curves and mean lifespan of fh-GAAs; daGS>UAS-Fdx1 RNAi flies. d, Male flies were either untreated or treated with various concentrations of RU486 during development from 0.1 to 1.6 μg ml−1, with a constant RU486 concentration of 10 μg ml−1 at the adult stage. e, Male flies were untreated or treated with 1.6 μg ml−1 RU486 during development and either untreated or treated with 10 μg ml−1 or 100 μg ml−1 at the adult stage (datasets for 0-0 and 1.6-10 conditions are similar in d and e). Labels Dev-Adult indicate RU486 concentrations (μg ml−1) in the fly food during development and adult stages. a,b, Dots (n = 6 except for daGAL4>+, n = 5) correspond to independent biological samples each obtained from 20 individuals. Mean values ± s.d. are indicated. a.u: arbitrary units. Two-sided non-parametric Mann–Whitney tests were used. (c–e) The total numbers of flies per condition and the p values obtained by log rank tests are provided in Extended Data Table 4. For each condition, the flies were housed in tubes by groups of mainly 28 to 30 individuals. For mean lifespan analysis, each dot corresponds to one tube (n = 5 in (c), n = 4 in (d,e) except for the 0–100 (n = 3) and the 1.6-0 treatments (n = 5)). Mean values ± s.d. are indicated. One-way Anova followed by Post-hoc Tukey analysis for multiple comparisons was used. Precise number of flies per tube and P values are provided in the source data. *P < 0.05, **P < 0.01, ***P < 0.001.
