Fig. 2: FIDA and ITC analyses of the interactions of FDX2 and FXN with the core ISC complex.
From: Cross-regulation of [2Fe–2S] cluster synthesis by ferredoxin-2 and frataxin
a–d, FIDA measurements showing the change in the apparent hydrodynamic radius (Rapp) of: FXNALC after titration of the (NIAU)2 complex (a), the preformed FXNALC–(NIAU)2 complex after titration of FDX2 (b), FDX2ALC after titration of the (NIAU)2 complex (c) and the preformed FDX2ALC–(NIAU)2 complex after titration of FXN (d). e–g, ITC measurements monitoring the interaction between: FXN and the (NIAU)2 complex (e), FDX2 and the (NIA)2 complex (f) and FDX2 and the (NIAU)2 complex (g). Heat of injection error bars correspond to unbiased NITPIC-assigned integration errors of thermal spikes. Data in a–d are mean ± s.d., n = 3 independent experiments, presented in Extended Data Table 1; data in e–g are representative experiments from n = 3 independent experiments with similar results, presented in Extended Data Table 2.
