Extended Data Fig. 7: Phenotypes of T cells in the spleens of Epor^flox/flox vs. Epor^ΔXcr1 mice and role of EPOR in cDC1-mediated cell-associated Ag-specific CD4⁺ T cell priming and proliferation and FOXP3⁺ T_reg induction.

a-e, Percentages and absolute numbers of CD4⁺ T cells (a), FOXP3⁺CD25⁺ T_regs in CD4⁺ T cells (b), CD44^highCD62L^low effector cells and CD44^lowCD62L^high naïve cells in CD4⁺ T cells (c), CD8⁺ T cells (d), and CD44^highCD62L^low effector cells and CD44^lowCD62L^high naïve cells in CD8⁺ T cells (e) in the spleens of Epor^ΔXcr1 and littermate Epor^flox/flox control mice with representative flow cytometric plots. a-e, Epor^flox/flox, n = 5; Epor^ΔXcr1, n = 5. f,g, Flow cytometry-based measurement of cell-associated Ag-specific CD4⁺ T cell immune response in the spleen following i.v. injection of apoptotic Act-mOVA thymocytes into mice of the indicated genotypes 1 day after i.v. injection of CTV-labeled naïve CD45.1⁺ OT-II cells. f, WT C57BL/6 (n = 5) and Batf3^−/− (n = 7). g, FOXP3⁺ T_reg induction in Epor^flox/flox and Epor^ΔXcr1 mice. Recombinant EPO or PBS was administered daily, from Day −3 to Day 4. +PBS: Epor^flox/flox (n = 5), Epor^ΔXcr1 (n = 5); +EPO: Epor^flox/flox (n = 5), Epor^ΔXcr1 (n = 5) mice. Data are shown from one experiment, representative of at least three independent experiments with similar results (a-e), or two independent experiments with similar results (f,g). Statistical analysis was performed using unpaired two-tailed Student’s t-test (a-f), or two-way ANOVA followed by Tukey’s multiple-comparison test (g). Data are mean ± s.e.m. (a-g). The diagrams in f,g were created in BioRender. Zhang, X. (2025) https://BioRender.com/bth22u6.

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