Extended Data Fig. 8: Epor-tdT expression on XCR1⁺ cDC1s in selected organs and tolerogenic phenotype of Epor-tdT⁺ migratory cDC1s in PLN.

a,b,c CCR7- and Batf3-dependent Epor-tdT expression on migratory cDCs in pLNs. Migratory cDCs were gated as CD11c^intMHCII^high from live-dead aqua⁻Lin⁻SiglecH⁻PDCA-1⁻EpCAM⁻ cells, and resident cDCs were gated as CD11c^highMHCII^int from live-dead aqua⁻Lin⁻SiglecH⁻PDCA-1⁻ cells. pLNs including inguinal, axillary, brachial, and superficial cervical LNs were combined for analysis by flow cytometry (a,b). Ccr7^−/−Epor^tdT/+, Batf3^−/−Epor^tdT/+ and WT C57BL/6 mice (a). Histogram overlay of Epor-tdT expression on migratory or resident cDCs from individual mouse strains (b). Epor-tdT expression on migratory cDCs from individual pLNs of Epor^tdT/+ mice (c). d, Epor-tdT expression on cDC1s obtained from the indicated organs in Zbtb46^GFP/+Epor^tdT/+ mice. cDCs were gated in CD45⁺ cells as CD11c⁺Zbtb46-GFP⁺, in which cDC1s were further gated as XCR1⁺CD103⁺. e, Flow cytometric analysis of tolerance associated cell-surface molecules on PLN Epor-tdT⁺ migratory cDC1s compared with Epor-tdT⁻ cDCs and resident cDCs with FMO serving as controls (n = 8). Data are representative of at least two independent experiments with similar results (a-e). Statistical analysis was performed using one-way ANOVA Tukey’s multiple-comparison test (e). Data are mean ± s.e.m. (e). The diagrams in a,e were created in BioRender. Zhang, X. (2025) https://BioRender.com/5sr2iny.

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