Extended Data Fig. 12: Loss of EPOR in cDC1s limits tumor growth and promotes immunogenic function of tumor Ag-carrying cDC1s in both TDLN and tumor.
From: Erythropoietin receptor on cDC1s dictates immune tolerance
a, Growth of MC38-OVAdim tumor cells implanted s.c. into Eporflox/flox (n = 8) and EporΔXcr1 mice (n = 9). b, Growth of B16F10-OVA tumor cells implanted s.c. into Eporflox/flox (n = 6) and EporΔXcr1 mice (n = 7). c, Experimental design for phenotyping tumor-Ag carrying ZsGreen+ cDC1s in tdLN and tumors in Eporflox/flox vs. EporΔXcr1 mice. B16F10-OVA-ZsGreen cells were implanted s.c. into EportdT/+ mice, and TDLNs and tumors were analyzed on day 9 after implantation. d, Flow cytometry analysis of Epor-tdT expression on ZsGreen+ migratory XCR1+SIRPα− cDC1s in tdLNs with summary graph of statistical quantification. Eporflox/flox (n = 7) and EporΔXcr1 (n = 8). e, Flow cytometry analysis of CD40, CD80 and CD86 expression on tumor infiltrating ZsGreen+ cDC1s with summary graph of statistical quantification. Eporflox/flox (n = 7) and EporΔXcr1 (n = 8) mice. (f-l) MC38-OVAdim tumors were s.c. implanted into Eporflox/flox (n = 8) and EporΔXcr1 (n = 7) and 10 days later TILs were analyzed. f. Percentages of CD45+ live immune cells and CD8+ or CD4+ T cells in CD45+ TILs. g, Frequency of OVA257-264-dextramer+CD8+ T cells among CD8+ T cells. h, Representative flow plots and quantification of CD8+ T cells expressing TIM-3 and PD-1. i, Representative flow plots and quantification of TCF1+TIM-3−CD8+ T cells. j, Representative histograms and quantification of perforin, granzyme-B, IFNγ and TNFα expression in tumor-infiltrating CD8+ T cells. k, Percentage of FOXP3+ Tregs in CD4+ T cells with representative flow plots (Left). Absolute number of Tregs (Right). l, Representative flow plots and percentages of T-bet+CXCR3+ Tregs in CD4+ FOXP3+ Tregs. (f-l). Data are shown from one experiment, representative of at least two independent experiments with similar results (a,b,d,e,f-l). Statistical analysis was performed using two-way ANOVA with Šídák’s multiple comparison test (a,b), or two-tailed unpaired Student’s t-test (d,e,f,g,i,j,k,l), or two-way ANOVA with Tukey’s multiple-comparison test (h). Data are mean ± s.e.m. (a,b,d,e-l). The diagram in c was created in BioRender. Zhang, X. (2025) https://BioRender.com/3jod9q7.
