Extended Data Fig. 3: Optimization of 3D suspension in vitro culture condition and quality classification of monkey embryoids from D17 to D25.
From: Modelling late gastrulation in stem cell-derived monkey embryo models
a, Comparison of different prolonged culture conditions of monkey embryoids from D17. Representative brightfield images of monkey embryoids on D25 were shown in the right. Scale bars, 200 μm. b, Developmental efficiency of in vitro-cultured monkey embryoids from D17 to D25 under different conditions were statistically analyzed. c, Representative images of monkey embryoids divided from three groups, optimal, suboptimal and medium group according to clear standards during in vitro culture from D17 to D25. N = 3 for each group to present. A, anterior; P, posterior. Scale bars, 100 μm. d, Statistical analysis of formation efficiency of each group from D17 to D25. n = 79 for Optimal group, n = 21 for Suboptimal group and n = Medium group from 3 independent experiments. e, Measurement of embryonic disc diameters of natural human embryos from CS8 (n = 8) and CS9 (n = 8) (Data from Carnegie stage, O’Rahilly et al. 1987) and monkey embryoids from D22 and D25, respectively (n = 15 for each group from 3 independent experiments). Data are presented as mean ± SEM. Each dot represents a sample. Two-sided unpaired t-test was used for D22 versus CS8-H and D25 versus CS9-H groups respectively, ns = P > 0.05 (P = 0.0604); *** = P ≤ 0.001. P = 0.0604 for D22 versus CS8-H group; P = 4.25E-06 for D25 versus CS9-H group. f, Representative images of monkey embryoids cultured beyond D25. N = 3 for each group to present. Scale bars, 100 μm.
