Extended Data Fig. 4: 3D analysis of monkey embryoids morphology.
From: Modelling late gastrulation in stem cell-derived monkey embryo models
a, Representative 3D images showing the typical lateral view of monkey embryoids from D17 to D25 by light sheet microscope analysis after immunostaining with OCT4 (red), OTX2 (green) and FOXA2 (gray) (Supplementary Videos 7–10). OCT4, a marker for epiblast; OTX2, a marker for neuroectoderm and endoderm; FOXA2, a marker for endoderm and some mesoderm. A, anterior; P, posterior. Scale bars, 200 μm. b, Monolayer from 3D morphology in (a) showing the typical lateral view of monkey embryoids from D17 to D25 by light sheet microscope analysis after immunostaining with OCT4 (red), OTX2 (green) and FOXA2 (gray). Two domains of OTX2, one that overlaps FOXA2 (ventral) indicating endoderm and another that overlaps OCT4 (dorsal) indicating NP. NP, neural plate; Endo, endoderm. Scale bars, 200 μm. c, Representative 3D images showing the typical dorsal view of monkey embryoids from D17 to D25 by light sheet microscope analysis after immunostaining with OCT4 (red), OTX2 (green) and FOXA2 (gray) (Supplementary Videos 7–10). Scale bars, 200 μm. d, Monolayer from 3D morphology in (b) showing the typical dorsal view of monkey embryoids from D17 to D25 by light sheet microscope analysis after immunostaining with OCT4 (red), OTX2 (green) and FOXA2 (gray). Scale bars, 200 μm. e, Representative 3D images showing the typical transversal view of monkey embryoids from D17 to D25 by light sheet microscope analysis after immunostaining with OCT4 (red), OTX2 (green) and FOXA2 (gray) (Supplementary Videos 7–10). NG, neural groove. D, dorsal; V, ventral. Scale bars, 200 μm. f, Schematic diagram of monkey embryoids at different stages from lateral, dorsal, and transversal views. Schematic was created using BioRender (https://biorender.com).
