Extended Data Fig. 5: Expansion of CD4 XDRTCCs following transplant and convergence of XDRTCC TCR nucleotide sequences to shared amino acid sequences post-transplant.

(A) Cumulative frequency of CD4 XDRTCCs and non-XDRTCCs at each timepoint. Pre-transplant frequencies were determined by normalizing the frequency of individual CD4 clones within the CD3⁺ pre-transplant population to the proportion of CD4⁺ cells within the CD3⁺ gate as determined by flow cytometry (Extended Data Fig. 8g). (B) Average number of nucleotide sequences encoding each unique amino acid sequence (nucleotide-per-aa) for XDRTCCs and non-XDRTCCs found in the pre-transplant and post-transplant peripheral blood. “Remainder” indicates clones that were identifiable as neither XDRTCCs nor non-XDRTCCs post-transplant. Statistical analysis was done using a two-sided Fisher’s exact test.