Extended Data Fig. 7: XDRTCCs from the direct or indirect pathway are detected across samples over time and some exhibit Tfh phenotype.
From: Physiology and immunology of a pig-to-human decedent kidney xenotransplant
(A). XDRTCC phenotypes are shown across samples. Phenotypes are determined by SingleR reference-based labeling using MonacoImmuneData. “CD4 helper” combines CD4 subsets Th1, Th2, and Th17. Representative CD8 clonotypes found across samples and their changes in phenotypes between the POD14L and POD33K samples are shown. One cell labeled “CD4 helper” was omitted for clarity. (B,C). XDRTCCs detected in the POD14L (clusters 0, 1, 6) and POD33 kidney biopsy (cluster 3) are primarily CD8 T cells from the direct pathway. (D). XDRTCCs with the indicated TCRB CDR3 sequences were detected in both the perinephric lymphocele at POD14 and within the kidney biopsy at POD33. (E). Cells in clusters 0 and 1 expressed CXCR5, CD154, and ICOS, consistent with a Tfh phenotype. One XDRTCC was identified among these Tfh.
