Extended Data Fig. 4: Analysis of activity and FFA composition in albumin filtrate.
From: Albumin orchestrates a natural host defence mechanism against mucormycosis
a, Outline of solid-phase extraction (SPE) procedure. GC-MS TIC chromatographs of derivatized fractions (F2–F4) and assessment of R. delemar growth (16 h). Scale bar, 50 µm. Caprylic acid concentrations: F2 = 194 µg/mL (1.34 mM), F3 = 15 µg/mL (104 µM), F4 = 0.1 µg/mL (0.7 µM). b, EI-MS spectrum of F2 (caprylic acid methyl ester) compared to NIST14.0 reference. Molecular ion (M+·, m/z 158) and characteristic fragments annotated. c, ESI(-)-TOF-MS of underivatized F2: [M–H]− at m/z 143.1079 with isotopic distribution; EC and RDB eq. provided. Data are a representative example of n = 2 independent experiments. d, Inhibition of R. delemar by increasing purified caprylic acid in 5% EtOH (n = 6 independent experiments). Data are mean ± s.d.
