Extended Data Fig. 3: Identification of spatially distinct LRA reactivity states.
From: Lesion-remote astrocytes govern microglia-mediated white matter repair
a, Quantification of LRA subtype markers for vGM3 (Thrsp, Arex) and WM4 (Ccn1, Glipr2) demonstrates region-enriched expression. LRA data from 7 and 28 dpi was combined for analysis. Statistical analysis was performed using students Two-sided t-test, *P ≤ 0.05, **P ≤ 0.002, ***P ≤ 0.0002, ****P ≤ 0.0001 (Thrsp/Arex: n = 3 uninjured,n = 6 injured, Ccn1: n = 3 uninjured, n = 4 injured, Glipr: n = 3 uninjured, n = 5 injured). b, Cell2Location abundance plots of regionally-restricted astrocyte molecular states across lesion-remote rostral spinal cord. Astrocyte cluster 11 (not shown) was not used for this analysis due to the small number of cells in this cluster. C, Cell2Location abundance plots of dGM2 astrocytes in lesion-remote rostral and caudal spinal cord. d-g, High magnification image of dGM2 marker Ak4 and Lair shows elevated expression in astrocytes (Gfap+/Aldoc+) in dorsal horn grey matter, but not white matter. h, High magnification image of vGM3 markers Thrsp and Arex shows lack of injury-reactive astrocyte expression in white matter. i, High magnification image of WM4 markers Glipr2 and Ccn1 show lack of injury-reactive astrocyte expression in grey matter. j, Mean expression of Gfap and Vim is used to identify hypertrophy-associated astrocyte molecular states (red bars) detected by snRNA-Seq expression. Differential expression testing for Gfap and Vim relative to regional healthy cluster by Wilcoxon rank sum test with Bonferri correction for multiple corrections. Graphs show mean ± SEM. In graphs of histological count or continuous data, colored data points represent the mean value for each biological replicate (individual mouse), while grey data points indicate replicate measurements from individual tissue sections. *P ≤ 0.05, ****P ≤ 0.0001. Exact P values can be found in source data. Scale bars: 10 µm.
