Extended Data Fig. 9: Tor1-Gad8 signaling axis modulates Raf1 levels and caffeine resistance.
From: Stress controls heterochromatin inheritance via histone H3 ubiquitylation
a, b, Western blot analysis of Raf1 under stress conditions for 16 h (a) or in the indicated strains (b). c, Real-time qPCR analysis of raf1 expression in WT or tor1Δ cells (data are presented as mean ± SD; n = 3 independent experiments, P value by two-tailed unpaired t test). d, WT, tor1Δ, and gad8Δ cells plated on 16 mM caffeine-containing medium. e, Quantification of caffeine resistant colonies (data are presented as mean ± SD; n = 3 independent experiments, P value by one-way ANOVA followed by Dunnett’s multiple comparisons test). f, Caffeine resistance in tor1Δ cells upon raf1-oe (Padh1-raf1). Serial dilutions were plated on nonselective (N/S) and 16 mM caffeine containing media. Data are representative of two independent experiments. Source data are provided.
