Extended Data Fig. 8: scRNA-sequencing analysis of IEC changes in response to acute nociceptor activation.
From: Neuro-epithelial circuits promote sensory convergence and intestinal immunity
a, Schematic of scRNA-sequencing analysis of the ileal epithelium upon chemogenetic nociceptor activation. b, UMAP of scRNA-seq data from IECs from mice in a. c, Dot plots showing the mean ileal expression of the indicated genes in each cluster. The dot size represents the proportion of cells in a cluster with the gene detected. d, Visualization of Slc34a2, Ccl25, Stmn1 and Olfm4 gene expression in Xenium dataset. Scale bar=100 µm. e-f, UMAPs of Slc34a2, Ccl25, Stmn1, Olfm4 and Fgfbp1 gene expression from the scRNA-seq analysis. g-h, Volcano plot showing differentially expressed genes (P < 0.05) in indicated IEC clusters. i, GO pathway analysis of the indicated cluster in TRPV1hM3Dq group compared to their littermate controls. Shown images are representative of 3 biological replicates in each group from 1 independent experiment in d. Statistics were calculated by two-tailed likelihood-ratio test used on pseudobulked expression values (g-h) or by one-tailed Fisher’s exact test (i). Multiple test correction was performed using the Benjamini-Hochberg procedure (g-i). The illustration in a was created in BioRender. Emanuel, E. (2025) https://biorender.com/b1xymo3.
