Extended Data Fig. 2: Gating strategies for immune cells and epithelial cells in the mouse mesenteric lymph nodes and intestines.

Gating strategy for different immune cells with surface markers. Lineage markers included: CD3ε, CD5, TCRγδ, CD11b, CD11c, B220, CD19, F4/80, FcεRIα and TER-119. a, Eosinophils were gated as Live CD45⁺ SiglecF⁺. b, ILC2s were gated as Live CD45⁺ Lineage⁻ CD127⁺ CD90.2⁺ KLRG1⁺ GATA3⁺. c, CD4⁺ T cells were gated as Live CD45⁺ CD3ε⁺ CD4⁺; and CD4⁺ Th2 cells were further gated as GATA3⁺ FoxP3⁻; CD4⁺ Th1 cells were further gated as T-bet⁺ FoxP3⁻. d, Epithelial cells were gated as Live CD45^−/lo EpCAM⁺ cells. Among those cells, tuft cells were gated as CD24^hi SiglecF⁺. For experiments using Lgr5^eGFP mice, non-tuft epithelial cells (ECs) were gated as CD24^–/lo SiglecF⁻. Among the non-tuft ECs, intestinal stem cells (ISCs) were gated as GFP^hi, progenitor cells were gated as GFP^lo, and other ECs were gated as GFP⁻.