Extended Data Fig. 3: Tuft cell-deficient mice exhibit defective type 2 immunity to Trichuris infection.
From: Neuro-epithelial circuits promote sensory convergence and intestinal immunity
a, Schematic of Trichuris infection in Pou2f3−/− mice (n = 4-5) compared to Pou2f3+/− littermate controls (n = 4). b-d, Representative flow cytometry plots (b), frequency (c) and total number (d) of Lineage– GATA3+ ILC2s in the mLNs. e, Frequency of GATA3+ CD4+ T cells in the mLNs. f-g, Representative histology and goblet cell quantification of the proximal colon by AB-PAS staining. Scale bar=50 µm. h, Trichuris worm counts from the ceca of Pou2f3−/− (n = 9) versus Pou2f3+/− littermates (n = 9). Data are representative of 4 independent experiments (c-e, g) or pooled from 2 experiments (h). Data are mean ± s.e.m. Statistics were calculated by unpaired two-tailed t-test. The illustration in a was created in BioRender. Emanuel, E. (2025) https://biorender.com/b1xymo3.
