Extended Data Fig. 2: piRNA pathway factors are euchromatic from initiation of expression.
From: A nowhere-to-hide mechanism ensures complete piRNA-directed DNA methylation
a-c, Representative seminiferous cords stained for HA (SPOCD1-HA) (a), MIWI2 (b) and DNMT3L (c), H3K4me3 and DAPI from Spocd1HA/+ (a) or wild-type mice (b-c) at the indicated developmental stages. Shown to the right are boxplots of Pearson’s correlation coefficients of SPOCD1 (a), MIWI2 (b) and DNMT3L (c) with H3K4me3 in gonocyte nuclei at the indicated developmental stages. MIWI2 and DNMT3L are not expressed at E14.5, so co-localization was not calculated for this developmental stage. Scale bars, 10 μm. Images in a through c are representative of n = 3 biological replicates. Box indicates interquartile range, central line represents the mean, and whiskers extend to median ± 1.5× the interquartile range or data limits, whichever is smaller.
