Extended Data Fig. 4: SPOCD1-K464A does not interact with TPR.
From: A nowhere-to-hide mechanism ensures complete piRNA-directed DNA methylation
a, Multiple sequence alignment of the SPOCD1 TFIIS-M domains from the indicated species. Red rectangles highlight lysine residues making direct crosslinks with TPR-M in the CL-MS data. Sequence identity conservation is shown by depth of colour. b, Representative Coomassie-stained gel image of n = 3 MBP pull-down experiments with the indicated recombinant mouse SPOCD1 and human TPR fragments. For uncropped source gel image, see Supplementary Fig. 1d.
