Extended Data Fig. 8: Rescue of LoF mutant D1R mediated by \({{\bf{GEM}}}_{{\bf{ago}}{\boldsymbol{-}}{\bf{PAM}}}^{{\bf{TM5}}{\boldsymbol{/}}{\bf{6}}{\boldsymbol{/}}{\bf{7}}}\). | Nature

Extended Data Fig. 8: Rescue of LoF mutant D1R mediated by \({{\bf{GEM}}}_{{\bf{ago}}{\boldsymbol{-}}{\bf{PAM}}}^{{\bf{TM5}}{\boldsymbol{/}}{\bf{6}}{\boldsymbol{/}}{\bf{7}}}\).

From: De novo design of GPCR exoframe modulators

Extended Data Fig. 8

a-f, Dose-response curves showing dopamine-induced cAMP accumulation in cells expressing various D1R LoF mutants rescued by \(\text{GE}{\text{M}}_{\text{ago}-\text{PAM}}^{\text{TM}5/6/7}\): D103A (a), L66F (b), D70A (c), F281R (d), N327A (e), and T37K (f). The experiments were conducted in the presence of low (light blue), medium (blue), or high (dark blue) concentrations of \(\text{GE}{\text{M}}_{\text{ago}-\text{PAM}}^{\text{TM}5/6/7}\). Wild-type D1R (D1R-WT, dashed grey line) and the mutant D1R alone (D1R-mut, yellow) are shown as controls. Values are shown as the mean ± SEM from three independent experiments performed in triplicate. A detailed statistical evaluation is provided in Extended Data Table 6.

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