Extended Data Fig. 5: Cryo-EM data processing and validation.

a-d, Results of \(\text{GE}{\text{M}}_{\text{anchor}}^{\text{TM}1/2/4}\)-D1R-G_s complex. a, Cryo-EM map. b, Cryo-EM map colored according to local resolution. c, Representative 2D averages. d, Fourier shell correlation (FSC) curve, the resolution was assessed by the Gold Standard of FSC = 0.143. e-h, Results of \(\text{GE}{\text{M}}_{\text{BAM}}^{\text{TM}3/4/5}\)-D1R-G_s complex. e, Cryo-EM map. f, Cryo-EM map colored according to local resolution. g, Representative 2D averages. h, FSC curve. i-l, Results of \(\text{GE}{\text{M}}_{\text{NAM}}^{\text{TM}5/6/7}\)-D1R-BRIL complex. i, Cryo-EM map. j, Cryo-EM map colored according to local resolution. k, Representative 2D averages. l, FSC curve. m-p, Results of \(\text{GE}{\text{M}}_{\text{ago}-\text{PAM}}^{\text{TM}5/6/7}\)-D1R-G_s complex. m, Cryo-EM map. n, Cryo-EM map colored according to local resolution. o, Representative 2D averages. p, FSC curve. q-t, Results of \(\text{GE}{\text{M}}_{\text{anchor}}^{\text{TM}1/2/4}\)-\(\text{GE}{\text{M}}_{\text{BAM}}^{\text{TM}3/4/5}\)-\(\text{GE}{\text{M}}_{\text{NAM}}^{\text{TM}5/6/7}\)-D1R-BRIL complex. q, Cryo-EM map. r, Cryo-EM map colored according to local resolution. s, Representative 2D averages. t, FSC curve. u-x, Results of\(\,\text{GE}{\text{M}}_{\text{anchor}}^{\text{TM}1/2/4}\)-\(\text{GE}{\text{M}}_{\text{BAM}}^{\text{TM}3/4/5}\)-\(\text{GE}{\text{M}}_{\text{ago}-\text{PAM}}^{\text{TM}5/6/7}\)-D1R-G_s complex. u, Cryo-EM map. v, Cryo-EM map colored according to local resolution. w, Representative 2D averages. x, FSC curve.