Extended Data Table 3 Influence of fused-GEM on cAMP accumulation and β-arrestin 2 recruitment mediated by D1R
- aGloSensor results of cAMP accumulation and BRET assay of β-arrestin 2 recruitment for D1R (WT and GEM binding) were normalized to the maximal response of WT D1R. Data are presented as means ± SEM from at least three independent experiments performed in technical triplicate. NSP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by one-way ANOVA followed by Dunnett’s post-test compared with WT D1R (P = 0.0459, = 0.3683, <0.0001 from top to bottom for pEC50 in the cAMP accumulation assay; P = 0.8062 for pEC50 in the β-arrestin 2 recruitment assay of D1R-\(\text{GE}{\text{M}}_{\text{anchor}}^{\text{TM}1/2/4}\); P = 0.7011, = 0.9877, <0.0001 from top to bottom for ΔpEC50 in the cAMP accumulation assay; P > 0.9999 for ΔpEC50 in the β-arrestin 2 recruitment assay of D1R-\(\text{GE}{\text{M}}_{\text{anchor}}^{\text{TM}1/2/4}\); P = 0.9907, > 0.9999, = 0.1965 from top to bottom for efficacy in the cAMP accumulation assay; P = 0.1301, <0.0001, <0.0001 from top to bottom for efficacy in the β-arrestin 2 recruitment assay).
- bThe efficacy is defined as the window between the maximal response (Emax) and the vehicle (no agonist). NR refers to no response (or response <10% WT) occurred as the concentration of ligand changes. ND, not determinable, basal activity was too high to determine an accurate EC50 value, which refers to cannot be established over the tested concentration range, such that an Emax was not reached and therefore span could not be calculated.
