Extended Data Table 6 Rescue of LoF mutant D1R mediated by \({{\bf{GEM}}}_{{\bf{ago\mbox{--}PAM}}}^{{\bf{TM5}}{\boldsymbol{/}}{\bf{6}}{\boldsymbol{/}}{\bf{7}}}\)

From: De novo design of GPCR exoframe modulators

  1. aGloSensor results of cAMP accumulation for D1R (WT, mutant and GEM binding) were normalized to the maximal response of WT D1R. Data are presented as means ± SEM from at least three independent experiments performed in technical triplicate. NSP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by one-way ANOVA followed by Dunnett’s post-test comparing each mutant to its corresponding D1R control without GEM (P < 0.0001, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, = 0.0004, <0.0001, <0.0001, <0.0001, <0.0001, <0.0001, = 0.0820, = 0.0150, = 0.0034, = 0.0148, = 0.0005, = 0.0002 from top to bottom).
  2. bThe relative response is defined as the activity of D1R stimulated by 100 μM dopamine with or without GEM.
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