Extended Data Fig. 8: Reward magnitude modulates the response amplitude of reward-approach neurons.

a, Schematic of a mouse running towards reward (bigger reward) versus incentive (smaller reward). b, Two example neurons showing stronger response when the mouse is running towards the reward (blue) vs incentive (red). c, Two example neurons showing similar responses when the mouse is running towards reward (blue) vs incentive (reward). In the bottom row of b,c, the solid line and shaded area show mean and s.e.m., respectively. d, The average calcium activity across reward-approach neurons is higher when the mouse (this example mouse #5) is approaching the reward compared to the incentive. Each point represents one recording session (n = 24 sessions). e, Similar analysis as d across mice. Each point is obtained by averaging across seven sessions (n = 7 mice). Bar graphs and error bars in d,e show mean ± s.e.m. The schematic in a was created using Affinity Designer.