Extended Data Fig. 6: Decreased numbers of YFP+ kidney resident macrophages after acute deletion of DHPS by in vivo tamoxifen administration in Dhps flox/flox CX3CR1-ERT2 Cre Rosa26eYFP vs Dhps +/+ (control) CX3CR1-ERT2 Cre Rosa26eYFP mice.
From: The transition from monocyte to tissue-resident macrophage requires DHPS
a, Representative immunofluorescence images of kidney from adult Dhps +/+ CX3CR1-ERT2 Cre Rosa26eYFP (control) and Dhps flox/flox CX3CR1-ERT2 Cre Rosa26eYFP mice 5 weeks after in vivo tamoxifen administration. CX3CR1-ERT2 Cre-targeted kidney resident macrophages were stained with fluorescently-tagged antibodies against GFP to detect YFP+ cells (CX3CR1-ERT2Cre). Nuclei were visualized by DAPI staining. b, Quantification of YFP+ kidney macrophages per unit area (mm2) in Dhps +/+ (control) and Dhpsflox/flox CX3CR1-ERT2 Cre Rosa26eYFP mice are shown. All images and graphs are representative of 3 mice per group. Quantification was performed across the entire tissue section and expressed as YFP+ cells per unit area. Data are mean ± s.d. Exact P-values are indicated. Illustration in a was created using BioRender (https://biorender.com).
