Extended Data Fig. 3: Frequency and absolute numbers of RTM and macrophage subpopulations in brain and spleen.
From: The transition from monocyte to tissue-resident macrophage requires DHPS
Macrophage subpopulations from Dhps+/+ Rosa26eYFP (control) or Dhps-ΔM Rosa26eYFP reporter mice were measured by flow cytometry in a, brain, and b, spleen. CD45 + YFP+ cells were gated on singlets and live cells. Representative plots and graphs summarize results of at least two independent experiments. Data are mean ± s.d. Exact P-values are indicated. Red boxes indicate RTM populations. Illustrations in a and b were created using BioRender (https://biorender.com).
