Extended Data Fig. 1: Neural characterization of the ENS and CNS in 3KL αS transgenic mice.

a,b, Confocal overview (a) and insert (b) images of s129p in ME of postmortem PD jejunum. c,d, Reconstructed 3D images (c) and quantification of myenteric GFAP and HUC/D volume (d) in duodenum of 3 mo WT vs 3KL, n = 5 animals/genotype and 4 ROI/ animal. 2 experiments, data analysed using unpaired t test. e, Heatmap showing total αS and s129p⁺ αS levels in lamina propria and myenteric plexus of 3 mo 3KL vs WT mice, obtained by DSP. n = 2, 2–3 ROI/Mouse. f,g, Confocal micrographs of total αS, s129p αS and choline acetyltransferase (ChAT) in WT vs 3KL DMV at 3 mo (f) and 6 mo (g). Insert in g shows an individual ChAT⁺ DMV cell. Scale bars = 50 μm, insert in g = 10 μm. h-k, quantification of punctate s129p⁺ αS (h,j) or total punctate αS (i,k) within ChAT⁺ DMV cells by number of puncta per ChAT⁺ cell (left) and mean %area covered per ChAT⁺ cell (right) of 3 mo (h,i) or 6 mo (j,k) WT vs 3KL. n = 4 mice (WT) and n = 3 mice (3KL), 53–107 ROIs per mouse (h, i) and n = 5 mice (WT) and n = 6 mice (3KL), 13–108 ROIs per mouse (j,k). 2 experiments, unpaired t test or Mann-Whitney test (j, left). Data are mean ± s.e.m.

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