Extended Data Fig. 4: Supplemental global proteomics volcano plots and substrate validation with DEG-47 in the presence and absence of 10 µM SB-405483.
From: Identification of an allosteric site on the E3 ligase adapter cereblon
(a–c) Volcano plots of MOLM-13 cells after 18 h incubation with (a) 10 nM DEG-47 and 10 µM SB-405483 (b) 10 µM SB-405483 and (c) 10 nM DEG-47. (d) Western blot analysis of AHRR, CESI, and TXNIP after treatment of MOLM-13 cells with SB-405483 for 18 h. (e–f) Volcano plots of MOLM-13 cells after 18 h incubation with 10 nM DEG-47 and 10 µM SB-405483 versus MOLM-13 cells treated with (e) 10 µM SB-405483 (f) 10 nM DEG-47 for 18 h. (g) Western blot analysis of Wee1 levels after treatment of MOLM-13 cells with 10 nM DEG-47 in the presence or absence of SB-405483 for 18 h. (h) Western blot analysis of WIZ levels with increasing concentration of DEG-47 with and without 10 µM SB-405483 for 18 h. All proteomics experiments were performed with n = 4 biologically independent samples. P-values by t-test (background) method. For uncropped Western blot images, see Supplementary Fig. 4.
