Extended Data Fig. 5: Spatial Transcriptomic analysis of cortical interneuron changes in Fezf2 mutants.
From: Pyramidal neurons proportionately alter cortical interneuron subtypes
a, Representative Slide-seq data on a single coronal brain sections of the SSp cortex from WT (left) and Fezf2 KO (right) mouse at P37. b-c, Aggregate Slide-seq data from multiple brain sections, showing the overall distribution of PNs in control (b) and Fezf2 KO (c) cortices. Sample sizes are presented as n = mice (from X ROIs). WT control: MO, n = 3 (4); SSp, n = 5 (8); VISp: n = 4 (4). Age: P28-37. Fezf2 KO: MO, n = 3 (3); SSp, n = 5 (6); VISp, n = 2 (4). Age: 4–6 weeks. d-e, Identification of deep-layer PVALB and SST interneuron subtypes within the aggregate control (d) and Fezf2 KO (e) datasets. (same dataset as in b-c). f, Ridge plots showing the distribution of selected PVALB and SST subtypes in the deep layers of control and Fezf2 KO brains, based on MERFISH data as in Fig. 2c.
