Extended Data Fig. 8: Spatial transcriptomics analysis of Bax removal in PVALB and SST interneurons.

a, Additional MERFISH data at P14 from different genotypes, complementing Fig. 3c. b, Quantification of selected interneuron subtype numbers in the SSp region (MERFISH data), normalized to deep-layer PN count. Values are not directly comparable to Slide-seq due to the higher spatial resolution and cell-calling recovery rate of MERFISH. No statistical test was performed due to limited sample size. n=mice (ROIs); Control: n = 2(3), Bax cKO: n = 1(2), Fezf2 KO: n = 3(3), Fezf2 KO_Bax cKO: n = 1(2). c, Proportion of selected PVALB and SST interneuron subtypes within total L5/6 PVALB and SST interneurons, based on Slide-seq data, used to justify pooling of heterozygous genotypes in control group. A detailed statistical justification for pooling is provided in Supplementary Note. Fezf2 WT: n = 5 (8); Fezf2 HET_Bax cHET: n = 2 (3); Fezf2 KO: n = 5 (6); Fezf2 KO_Bax cHET: n = 2 (4). These data are included in Figs. 2d and 3d. d, Aggregate Slide-seq maps of coronal sections from Bax cKO and Fezf2 KO_Bax cKO mice, highlighting selected PVALB and SST interneuron subtypes identified in L5/6. Bax cKO: n = 3 (5); Fezf2 KO_Bax cKO: n = 3 (5). Age range: P28-33. e, Quantification of selected PVALB and SST subtypes (Slide-seq data), normalized to L5/6 PN counts, comparing control (n = 7 mice, 11 ROIs) and Bax cKO (n = 3 mice, 5 ROIs). f, Quantification of selected PVALB and SST subtypes (Slide-seq data), normalized to L5/6 PN counts, comparing between Fezf2 KO (n = 7 mice, 10 ROIs) and Fezf2 KO_Bax cKO (n = 3 mice, 4 ROIs). Boxplots: center line, median; box limits, 25th–75th percentiles; whiskers, 1.5xIQR. For c,e,f, LMM with animal ID as random effect. n.s., not significant, P ≥ 0.05; * P < 0.05; ** P < 0.01. Exact sample genotypes, sample ages, statistical model details and P values are provided in Supplementary Tables 2 and 3.

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