Extended Data Fig. 9: Additional data related to key findings in Fig. 4.
From: Convergent evolution of scavenger cell development at brain borders
a, HCR in situ hybridization staining of zebrafish (Danio rerio) brain section, showing expression of prox1a (green) and cell nuclei (DAPI, blue). n = 3 sections from n = 3 animals analysed. b, HCR in situ hybridization staining of axolotl (Ambystoma mexicanum) brain section, showing expression of flt4 (magenta) and cell nuclei (DAPI, blue). n = 3 sections from n = 3 animals analysed. c, HCR in situ hybridization staining of mouse (Mus musculus) brain section, showing expression of osr2 (green), flt4 (magenta) and cell nuclei (DAPI, blue). White dotted line marks leptomeningeal border. n = 3 sections from n = 3 animals analysed. d, HCR in situ hybridization staining of axolotl (Ambystoma mexicanum) whole spinal cord section, showing expression of csf1r (red) and cell nuclei (DAPI, blue). n = 3 sections from n = 3 animals analysed. e, HCR in situ hybridization staining of axolotl (Ambystoma mexicanum) whole brain section, showing expression of csf1r (magenta) and cell nuclei (DAPI, blue). n = 3 sections from n = 3 animals analysed. f, HCR in situ hybridization staining of mouse (Mus musculus) brain section, showing expression of prox1 (green), flt4 (magenta) and cell nuclei (DAPI, blue). n = 3 sections from n = 3 animals analysed. g, HCR in situ hybridization staining of adult fat-tailed dunnart (Sminthopsis crassicaudata) intestine with osr2 (green) and nuclei (DAPI, blue). This confirmed that the fat-tailed dunnart osr2 HCR probe works, despite not staining cells in the meninges. Representative of n = 3 biological replicates, and at least 3 technical replicates were imaged for each shown image. h, HCR in situ hybridization staining of E12.5 mouse embryo (Mus musculus) forelimb with osr2 (green) and nuclei (DAPI, blue). This confirmed that the mouse osr2 HCR probe works, despite not staining cells in the meninges Representative of n = 3 biological replicates, and at least 3 technical replicates were imaged for each shown image. i, Schematic depiction of the cellular composition of mammalian (left) and non-mammalian vertebrate (based on zebrafish, right) meninges. a-h, Scale bar 100 μm.
