Extended Data Fig. 7: Subtypes of PIEZO2-positive vagal sensory neurons that are dispensable for heartbeat-coupled responses.
From: Vagal blood volume receptors compensate for haemorrhage and posture change
a, UMAP plots depicting expression (natural log scale) of Phox2b, Piezo2, P2ry1, Glp1r, Pvalb and Oxtr based on a previously published vagal/glossopharyngeal cell atlas16. b, DTR immunostaining of vagal ganglia from P2ry1-ires-Cre; loxP-DTR, Pvalb-ires-Cre; loxP-DTR, Oxtr-ires-Cre; loxP-DTR and Glp1r-ires-Cre mice (left to right: 10, 7, 7, 6 replicates) that received no injection (top) or unilateral DT injection (bottom), scale bars: 500 µm. c, Representative (from replicates above) and simultaneously recorded electrocardiograms (ECG) and vagal nerve activity traces from the vagus nerve trunk corresponding to DT-injected ganglia of mice in b. d, Quantification of heartbeat-coupled vagal nerve trunk activity and intravenous saline infusion-induced vagal nerve trunk activity, mean ± sem, n = 12, 10, 7, 7, 6 mice, WT mice are the same reported in 3f and 3h. No statistical significance observed (p > .05) or **p = .0098 by a one-way ANOVA compared to wild-type (WT) with a Bonferroni correction for multiple comparisons.
