Extended Data Fig. 6: Effects of cellular sulfation and sulfatases on csRNP clustering.
From: GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling
a. Representative zoom-out images of Fig. 2c. Scale bar, 10 µm. 3 independent experiments were performed. b. Sequence alignment of partial NDST1, HS6ST1, and HS2ST1 coding sequences from wild-type (WT) and the indicated knock-out (KO) U2OS cell lines. Protospacer adjacent motif (PAM), green; sgRNA target, blue; mutated sequence, red. c. Western blot analysis of whole cell lysate from WT, NDST1, HS6ST1, and HS2ST1 KO U2OS cells. 2 independent experiments were performed. d. Representative images of WT and NaCl or NaClO3 treated U2OS cells stained with 10E4, Siglec-11, 9D5, anti-DDX21, and anti-hnRNP-U (all in red). Scale bar, 10 µm. 3 independent experiments were performed. e. Representative images of WT and NaCl or NaClO3 treated U2OS cells stained with 3G10 (red). Scale bar, 10 µm. 1 independent experiment was performed. f. Representative images of mEmerald-Sufl1 and mEmerald-Sulf2 stably overexpressing U2OS cells. Scale bar, 10 µm. 2 independent experiments were performed. g. Western blot analysis of whole cell lysate isolated from mEmerald-Sufl1 and mEmerald-Sulf2 stably overexpressing U2OS cells. 2 independent experiments were performed.
