Extended Data Fig. 7: Live cell labeling of HS-csRNP clusters in HUVECs and keratinocytes with RNase and heparinase treatment.
From: GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling
a. Representative images of HUVECs treated with the RNase pool or heparinase pool, and stained live with 10E4, Siglec-11, 9D5, anti-DDX21, and anti-hnRNP-U (all in red). Scale bar, 10 µm. b. Representative images of keratinocytes with the RNase pool or heparinase pool, and stained live with 10E4, Siglec-11, 9D5, anti-DDX21, and anti-hnRNP-U (all in red). Scale bar, 10 µm. c. Quantification of 10E4, Siglec-11, 9D5, cs-DDX21, and cs-hnRNP-U intensity per cell from 3 independent staining experiments on keratinocytes. Data are mean ± s.e.m.
