Extended Data Fig. 11: Activity of VEGF-A165 HS WT and HS(R/K) in zebrafish.
From: GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling
a. Western blot analysis of whole cell lysate isolated from zebrafish. b. Representative images of fli1a:EGFP zebrafish larvae at 36 h post fertilization (hpf) with the indicated injection of mRNA. DNA was stained with DAPI (blue). Scale bar, 0.3 mm. c. Quantification of GFP-positive endothelial cells numbers in the hatched box is calculated across three independent experiments. Boxes plots show the median, the 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). Statistical assessment was performed with a two-sided Student's t-test and P values are shown. d. Representative images of zebrafish larvae at 36 hpf with the indicated injection of mRNA. Scale bar, 0.3 mm. e. Quantification of larvae length in (C) is calculated across three independent experiments. Boxes plots show the median, the 25th and 75th percentiles (bounds of the box), and minimum and maximum values (whiskers). Statistical assessment was performed with a two-sided Student's t-test and P values are shown. f. Western blot analysis of whole cell lysate isolated from zebrafish. g. RT-PCR analyses of Wnt3a-target gene expression in zebrafish cells post fertilization with the indicated injection of mRNA. Statistical assessment was performed with a two-sided Student’s t-test and P values are shown. Data are mean ± s.e.m.
