Fig. 2: csRNPs are dependent on intact HS chains.
From: GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling
a, Representative images of U2OS cells treated with a heparinase pool for 30 min, recovering for the indicated times and co-stained with 10E4 (cyan), Siglec-11 (yellow) and 9D5 (magenta). An enlargement of the hatched box is shown, and a bright field is shown to represent the outline of the cell membrane. Three independent experiments were performed. b, Schematic of a HSPG with the regions of activity for the various enzymes and HS oligos perturbed in the following experiments. 2S, 2-O-sulfation; 6S, 6-O-sulfation; NS, N-sulfation. c, Representative images of WT, NDST1-KO-treated, HS6ST1-KO-treated, HS2ST1-KO-treated, Sulf1 stably overexpressing (OE)-treated, Sulf2 stably OE-treated, Tega HS #09-treated and Tega HS #37-treated U2OS cells stained with 10E4, Siglec-11, 9D5, anti-DDX21 and anti-hnRNP-U (all in red) separately. d, Quantification of data in panel c with the number of cells counted from three independent experiments. Data are mean ± s.e.m.
