Extended Data Fig. 7: Structural differences between Gi and Gs.
From: Non-equilibrium snapshots of ligand efficacy at the μ-opioid receptor
a, Comparison of the linker I region of Gs in the GTP-bound, cryo-EM frame 20 state (PDB: 8GGF) and GTP-analogue bound crystal structure (PDB: 1AZT). b, MD simulation AHD–RHD distance results for the adrenergic receptor–Gs complex and MOR–Gi complex with the linker I and Y69 swapped to match Gs, five independent trajectories each starting from either the AHD-closed or the AHD-open conformation. AHD–RHD distances for the open and closed conformations are shown as lines. c, Comparison of Gi in the G-ACT-2 state with Gs in the GTP-bound, cryo-EM frame 20 state (PDB: 8GGF, left) and GTP-analogue-bound crystal structure (PDB: 1AZT, right).
