Extended Data Fig. 7: Behavioural effects of chemogenetic manipulation of astrocyte Ca2+ via hM3Dq-actuation.
From: Astrocytes enable amygdala neural representations supporting memory
a, Optical fibre tip placement map for cyto-GCaMP6f in vivo astrocyte Ca2+ fibre photometry following pre-extinction CNO in animals expressing hM3Dq or a mCherry control virus. b, Population CS-related Ca2+ activity during early extinction (E-Ext, first 5 trials of extinction session 1), late extinction (L-Ext, last 5 trials of extinction session 2), extinction retrieval (E-Ret), and fear renewal (F-Ren) (Control n = 7, hM3Dq n = 9 mice). c, Quantification of CS-related Ca2+ activity shown in panel b (2-way repeated measures ANOVA and Šídák’s post hoc tests, during E-Ext *P < 0.0001, L-Ext *P = 0.0072, F-Ren *P = 0.0435, Control n = 6–7, hM3Dq n = 9 mice/stage). d, Effects of pre-test 3 mg/kg CNO on freezing levels (left) at baseline (base) and after exposure to footshock, with associated flinching scores (right), in animals expressing hM3Dq or a mCherry control virus (2-tailed unpaired t-test, P > 0.05, Control n = 6, hM3Dq n = 6 mice). e, Effects of 3 mg/kg CNO prior to fear conditioning on freezing levels during F-Con, Ext, E-Ret, and F-Ren in animals expressing hM3Dq or a mCherry control virus (2-way repeated measures ANOVA, Šídák’s or Fisher’s LSD post hoc tests, P > 0.05, Control n = 11, hM3Dq n = 12 mice). f, Effects of 3 mg/kg CNO immediately after F-Con on freezing levels during F-Con, Ext, E-Ret, and F-Ren in animals expressing hM3Dq or a mCherry control virus (repeated measures 2-way ANOVA, P > 0.05, Šídák’s or Fisher’s LSD post hoc tests, Control n = 11, hM3Dq n = 13 mice). g, Effects of 3 mg/kg CNO on novel open field total distance and centre time (2-tailed unpaired t-tests, P > 0.05, Control n = 14, hM3Dq n = 17 mice). h, Effects of 3 mg/kg CNO on elevated plus-maze total distance and open arm time (2-tailed unpaired t-tests, P > 0.05, Control n = 14, hM3Dq n = 17 mice). i, Effects of 3 mg/kg CNO on light-dark exploration test total distance and dark compartment time (2-tailed unpaired t-tests, P > 0.05, Control n = 17, hM3Dq n = 18 mice). j, Effects of pre-extinction 3 mg/kg CNO or Veh on freezing levels during F-Con, Ext, E-Ret, and F-Ren in animals expressing mCherry control virus (2-way repeated measures ANOVA, Šídák’s or Fisher’s LSD post hoc tests, Ext block 3 *P = 0.0424, Veh n = 10, CNO n = 10 mice). k, Effect of pre-extinction vehicle (Veh) administration on freezing levels during F-Con, Ext, E-Ret, and F-Ren in animals expressing hM3Dq or a mCherry control virus (2-way repeated measures ANOVA, Šídák’s or Fisher’s LSD post hoc tests, P > 0.05, Control n = 11, hM3Dq n = 11 mice). l, Effects of Veh administration on novel open field total distance and centre time (2-tailed unpaired t-tests, P > 0.05, Control n = 11, hM3Dq n = 11 mice). m, Effects of Veh administration on elevated plus-maze total distance and open arm time (2-tailed unpaired t-tests, P > 0.05, Control n = 11, hM3Dq n = 11 mice). n, Effects of Veh administration on light-dark exploration test total distance and dark compartment time (2-tailed unpaired t-tests, P > 0.05, Control n = 11, hM3Dq n = 11 mice). Horizontal lines above traces in b denote permutation test-determined significant difference from chance for hM3Dq (yellow) and Control (black) or significant difference between groups (orange). Fluorescence values z-scored to the 5-s pre-CS period. Data mean ± SEM.
