Extended Data Fig. 1: Fibre photometry measurement of astrocyte Ca2+ correlates of fear.
From: Astrocytes enable amygdala neural representations supporting memory
a, Representative GCaMP expression in astrocytes (GFAP) and neurons (NeuN), scale bar: 20 μm. b, Optical fibre tip location for cyto-GCaMP6f in vivo astrocyte Ca2+ fibre photometry. c, AUC quantification CS-related Ca2+ activity during each 5-CS trial-block of extinction session 1 (Ext 1) and 2 (Ext 2) shown in panel d (1-way repeated measures ANOVA and Šídák’s post hoc tests, E-Ext (Ext1 block 1) vs L-Ext (Ext2 block 5) *P = 0.0003, n = 17 mice/stage. e, Relationship between AUC of CS-related Ca2+ transients and freezing across test-sessions (2-tailed Pearson’s correlation, P = 0.0551, n = 14–17 mice). f, Heatmaps for CS-related Ca2+ activity (left) and EzTrack-detected freezing (right) across test phases (n = 14 mice). g, Ca2+ activity aligned to freezing and movement during early (E-Ext, first 5 trials of extinction session 1) and late (L-Ext, last 5 trials of extinction session 2) extinction (n = 133–135 onset episodes/stage, n = 158–200 offset episodes/stage, 14 mice). h, Example fibre placement and expression of the membrane-targeted Ca2+ indicator lck-GCaMP6f in astrocytes (GFAP) and neurons (NeuN) (2-tailed paired t-test, P < 0.0001*, n = 13 sections/6 mice) (scale bars: 200 μm (left), 10 μm (right). i, Optical fibre tip location for lck-GCaMP6f in vivo astrocyte Ca2+ fibre photometry. j, Population CS-related and shock-related Ca2+ activity during F-Con, with 3-trial-wise heatmap below (n = 11 mice). k, Population CS-related Ca2+ activity during E-Ext, L-Ext, E-Ret, and F-Ren, with 5-trial-wise heatmaps below (n = 10–11 mice/stage). l, AUC quantification CS-related Ca2+ activity shown in panel k (1-way repeated measures ANOVA and Šídák’s post hoc tests, E-Ext vs L-Ext *P = 0.0365, E-Ext vs E-Ret *P = 0.0003, E-Ret vs F-Ren *P = 0.0121, n = 10–11 mice/stage). Horizontal lines above traces in d,g,j,k denote permutation test-determined significant difference from chance. Fluorescence values z-scored to the 5-s pre-CS, pre-freezing or pre-movement periods. Data mean ± SEM.
