Extended Data Fig. 8: The C-terminal domain of Rip1_Eco mediates interaction with ST and determines phage specificity.

a, Schematic of the Rip1_Eco-ST interface showing phenylalanine 138 (F138) in Rip1_Eco, which was mutated to glutamate (F138E). b, Tenfold serial dilutions of indicated phages plated on various MG1655 lawns. Data are representative of three biological replicates. c, Coomassie-stained SDS-PAGE gels showing co-purification of untagged Rip1_Eco (F138E) with ST^WT-His₆. Data are representative of three biological replicates. d, Tenfold serial dilutions of indicated phages plated on different MG1655 or PA14 lawns. MG1655 EV and Rip1_Eco lawns are identical to those presented in panel b. Data are representative of three biological replicates.