Extended Data Fig. 3: Rip1Eco targets the small terminase and portal proteins.
From: A pore-forming antiphage defence is activated by oligomeric phage proteins
a, Tenfold serial dilutions of WT and defence escape mutant phages plated on MG1655 lawns with or without Rip1Eco. Data are shown as plaque-forming units (PFU) per mL and are representative of three biological replicates. b, MUSCLE sequence alignment of STWT from T6 and mutant ST variants from escape mutants of phages T4 and T6. The alignment was visualized using Jalview (v2.11.4.1), and residues were colored using the Clustal color scheme. Residues that are mutated in the escape mutants are labeled with black triangles. c, EOP of phage T6 on lawns expressing untagged Rip1Eco, N- or C-terminal Histidine-tagged proteins. Bars show the mean ± SD (n = 3 biologically independent replicates). Each replicate is shown as an individual dot, and asterisks show statistically significant differences relative to plasmid control (*P value < 0.05 (unpaired two-tailed t-test)). ns = not significant; P values = 0.0101 for Rip1Eco. d, Coomassie-stained SDS-PAGE gel showing co-purification of untagged Rip1Eco with STWT-His6. Data are representative of three biological replicates. Source Data are provided.
