Extended Data Fig. 11: SLAMF6 blockade reinvigorates chronically stimulated human T cells.
From: SLAMF6 as a drug-targetable suppressor of T cell immunity against cancer
Human CD8+ T cells lacking or not SLAMF6 were repeatedly stimulated with CD3 mAb OKT3 (3 μg mL−1) and IL-2 (10 ng mL−1). a, Flow cytometry analyses showing intracellular accumulation of IFN-γ and TNF-α in WT and SLAMF6−/− CD8+ T cells following re-stimulation with CD3 mAb OKT3 in the presence of the indicated SLAMF6 mAbs. n = 3 donors. b,c, Expression of Tim-3 and TCF-1 (b), or PD-1, Tox or LAG-3 (c) was determined by flow cytometry. n = 3 donors (b,c). Each symbol in a–c represents an individual blood donor; error bars represent mean ± s.d. For statistical analyses, two-sided unpaired Student’s t-test were used in a-c. Numbers above data points represent p values. Data in a-c are pooled from 3 independent experiments.
