Extended Data Fig. 12: SLAMF6 expression in tumour-infiltrating human CD8⁺ T cells.

a, Left, UMAP visualization of clusters (“c”) of tumour-infiltrating human CD8⁺ T cells (n = 76,833) coloured by cell type annotation. Clusters are numbered from 0 to 13 and identified according to the cell subsets they represent. The clusters corresponding to progenitor exhausted T cells (Tpex; c7) and non-progenitor exhausted T cells (Tex; c1) are highlighted. Tn, naïve-like T cells; Tn_TCF7, naïve-like T cells expressing TCF7, which encodes TCF-1; t-Teff, transitional effector T cells; Teff_KLRG1, effector T cells expressing KLRG1; Teff_CD244, effector T cells expressing CD244; Teff_SEMA4A, effector T cells expressing SEMA4A; Tcm, central memory T cells; Trm, resident memory T cells; Tstr, stress-response T cells; Tisg, interferon response T cells; Tsen, senescent T cells. Middle, UMAP visualization of SLAMF6 expression in the various clusters is indicated by colour intensity. Right, Dot plot showing SLAMF6 expression across clusters. Dot size represents the percentage of cells expressing SLAMF6, and colour intensity indicates mean normalized expression level. b, Left, Dot plot displaying expression of exhaustion-related markers (SLAMF6, TCF7, IL7R, PDCD1 (which encodes PD-1), HAVCR2 (which encodes Tim-3), ENTPD1, GZMB, GNLY, LAG3 (which encodes LAG-3)) in Tpex and Tex CD8⁺ T cells. Dot size represents percentage of cells expressing each RNA, and colour intensity shows scaled expression levels. Middle, UMAP visualization of exhausted CD8⁺ T cell subset (Tpex+Tex; n = 18,232 cells) with Tpex (blue) and Tex (red) identified by different colours. Right, Feature plots of key exhaustion markers and SLAMF6 across exhausted CD8⁺ T cells, with colour intensity showing normalized expression (Norm. exp.).