Extended Data Fig. 1: T cell markers and subset frequencies are comparable between WT and Slamf6^−/− mice.

a, Flow cytometry analyses of various cell surface markers on CD4⁺CD8⁻ and CD4⁻CD8⁺ thymocytes from WT and Slamf6^−/− mice. n = 4 (WT and Slamf6^−/−). b, Same as (a), except that expression of CD5, CD44 and CD25 was evaluated on CD4⁺CD8⁻, CD4⁻CD8⁺, CD4⁻CD8⁻ and CD4⁺CD8⁺ thymocytes. n = 4 (WT and Slamf6^−/−). c, Total thymocyte numbers, as well as proportions and numbers of CD4⁺ and CD8⁺ T cells in thymus from WT and Slamf6^−/− mice were determined. n = 4 (WT and Slamf6^−/−). d, As in a, except that splenic T cells were analysed. n = 4 (WT and Slamf6^−/−). e, As in c, except that splenic T cells were analysed. f,g, Proportions and numbers of regulatory T cells (Tregs) (f) and invariant (i) NKT cells (g) in spleen of WT and Slamf6^−/− mice. n = 4 (WT and Slamf6^−/−). h, Proportions and numbers of naïve (CD62L^hiCD44^lo), central memory (T_CM; CD62L^hiCD44^hi) and effector-memory (T_EM; CD62L^loCD44^hi) in CD4⁺ or CD8⁺ T cells from spleen of WT and Slamf6^−/− mice. n = 4 (WT and Slamf6^−/−). i, Flow cytometry analyses of the levels of SLAMF6 on subsets of splenic CD4⁺ or CD8⁺ T cells from WT and Slamf6^−/− mice. n = 3 (WT and Slamf6^−/−). j, Same as (i), except that the frequency of PD-1⁺ cells was analysed. n = 3 (WT and Slamf6^−/−). Each symbol in a-j represents an individual mouse; error bars represent mean ± s.d. Numbers above data points represent p values. Two-sided unpaired Student’s t-test (a-j). n = 4 mice (a-h) or n = 3 mice (i-j) from 3 independent experiments.

Source Data