Extended Data Fig. 5: Hepatic GPNMB and circulating G-ECD are positively associated with MASH severity.
(a to c) WT mice were fed a ND for 6 months or an AMLN diet for 3 or 6 months. Hepatic mRNA and protein expression of GPNMB and serum levels of G-ECD (n = 8 mice per group). (d) Spearman’s rank correlation coefficient analysis of hepatic GPNMB expression (log2) with MAS score and Pearson correlation coefficient analysis with serum ALT and AST (GSE89632). (e) Circulating proteomics of G-ECD in healthy individuals and patients with advanced MASLD without T2DM (Female: health n = 25, MASLD n = 3; male: health n = 36, MASLD n = 11). Data are presented as mean ± SEM (a, c, e). Unpaired two-tailed Student’s t-test (e) and one-way ANOVA with Tukey’s multiple-comparison test (a, c).
